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The windows of opportunity determined, are discussed in the context of empirical data on crude oil degradation in petroleum reservoirs and used to assess the relative importance of thermodynamics relative to biological factors in determining the routes of methanogenic crude oil degradation, observed in nature.Effect of chain length on the thermodynamics of anaerobic alkane degradationThe effect of chain length on the free energy yield of methanogenic degradation of alkanes was investigated to evaluate if inferences made using a single model alkane would be generally applicable across all alkanes.Thermodynamic calculations for all alkanes in the range C8 demonstrated that conversion to methane was exergonic.